Take Away Elecives In Schools - The aim of the present research is to study the effect of pH values on the sperm rheotaxis properties. Semen collected from bulls was diluted with SOF medium (). pH of the medium was adjusted using a digital pH meter to the following pH values: , , , , , All kinetic parameter . This indicates that rheotaxis could play an essential role in the long distance navigation of bull sperm through the female reproductive tract to the oocyte (Miki et. al, ). This finding is substantiated by a similar finding in studies by Kantsler et. al show more content al, ). We study rheotaxis of bull sperm inside microchannels to characterize the effects of flow and wall shape on sperm swimming behavior. We found that a large percentage of sperm cells, 80 to 84%, exhibited positive rheotaxis (sperm cells swimming against the flow) within flow velocities of 33 to μm s −zuncojp.somee.com cells were also found to reverse their swimming direction when the liquid flow. Staining Your Teeth Research Paper

Frederick Douglass An American Slave Rhetorical Analysis - Positive rheotaxis (PR) is the ability of sperm to swim against the flow. Our results show that PR is prevalent in bull sperm, which confirms rheotaxis as a strong mechanism for guiding sperm to the oocyte. Sperm can swim against secretions flowing downstream the female genital tract after coitus to reach the oocyte. Rheotaxis of sperm using a microfluidic device was explored in human, mice, and bull. However, the rheotaxis of ram sperm and its role in fertility are unknown. Herein, we described the sperm Estimated Reading Time: 8 mins. The aim of the present research is to study the effect of pH values on the sperm rheotaxis properties. Semen collected from bulls was diluted with SOF medium (). pH of the medium was adjusted using a digital pH meter to the following pH values: , , , , , Was The British Empire A Good Essay

The Kite Runner U Shaped Structure Analysis - In vitro sperm rheotaxis is affected by fluid flow velocity, shear stress and fluid viscosity 13,Increased fluid flow velocity and shear stress cause more sperm to show rheotactic behaivour. Interestingly, human and ram sperm did display rheotaxis when hyperactivated, similar to results observed with bull sperm as described by Kantsler, et al. 2 and Johnson, et al. Rheotaxis-based separation of sperm with progressive motility using a microfluidic corral system Meisam Zaferania, Soon Hon Cheongb, and Alireza Abbaspourrada,1 aDepartment of Food Science, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY ; and bDepartment of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY Essay On Mexican Drug War

The Journey Of Self-Leadership - sperm separation; medical infertility treatment; microfluidic; rheotaxis; Sperm motility is required for sperm to traverse the female genital tract, reach the site of fertilization, and penetrate the cumulus extracellular matrix around the oocyte and zona pellucida (1, 2).According to clinical reports, 10% of couples worldwide are infertile (3, 4), and almost half of these infertility cases. Rheotaxis of sperm using a microfluidic device was explored in human, mice and bull. However, the rheotaxis of ram sperm and its role in fertility are unknown. Herein, we described the sperm rheotaxis in ram using microfluidic devices and focused on rheotaxis as potential markers of in vivo fertilit . Rheotaxis of sperm using a microfluidic device was explored in human, mice and bull. However, the rheotaxis of ram sperm and its role in fertility are unknown. Herein, we described the sperm rheotaxis in ram using microfluidic devices and focused on rheotaxis as potential markers of in vivo fertility. Similarities Between Freud And Freyd

when does macbeth kill duncan - Request PDF | Effect of pH on rheotaxis of bull sperm using microfluidics | The aim of the present research is to study the effect of pH values on the sperm rheotaxis properties. Semen collected. Abstract. We study rheotaxis of bull sperm inside microchannels to characterize the effects of flow and wall shape on sperm swimming behavior. We found that a large percentage of sperm cells, 80 to 84%, exhibited positive rheotaxis (sperm cells swimming against the flow) within flow velocities of 33 to μm sSperm cells were also found to reverse their swimming direction when the liquid Estimated Reading Time: 11 mins. We study rheotaxis of bull sperm inside microchannels to characterize the effects of flow and wall shape on sperm swimming behavior. We found that a large percentage of sperm cells, 80 to 84%, exhibited positive rheotaxis (sperm cells swimming against the flow) within flow velocities of 33 to μm s −1. Sperm cells were also found to reverse their swimming direction when the liquid flow. Feminism In The 20th Century

Seismicity Patterns - the first quantitative study of mammalian sperm rheotaxis, using microfluidic devices to investigate systematically swimming of human and bull sperm over a range of physiologically relevant shear rates and viscosities. Our measurements show that the interplay of fluid shear, steric surface-. El-Sherry TM, Elsayed M, Abdelhafez HK, Abdelgawad M () Characterization of rheotaxis of bull sperm using microfluidics. Integr Biol (Camb) 6: – Crossref PubMed Web of . Sperm selection based on rheotaxis and thermotaxis (SSRT) platform. This study introduced the SSRT platform described in Fig. 1 that utilized an apparatus for prefreeze semen processing. It consists of a conical cylinder that is made of glass and a rotating mixer (Front Lab FLOSS) attached with a custom-made rotating two-blade paddle of closely controlled dimension and shape, which is made. Learning To Be Independent In College Essay

hans riegel, sr. - 1. The response of human and bull spermatozoa to vertical velocity gradients has been examined, the fluid flow being in horizontal planes. 2. Dead human and bull spermatozoa point downstream (negative rheotaxis) in the top half of a parabolic velocity gradient and upstream (positive rheotaxis) in . The tendency of mammalian sperm (bull and human) to swim from the center towards boundaries was previously observed in vitro in microfluidic channels (El-Sherry et al., ; Previous work on sperm rheotaxis has shown that the swimming velocity of sperm increases in response to an increase of the fluid flow velocity within a certain range. Because sperm capacitation can vary with respect to position and time in the female reproductive tract, we evaluated sperm rheotaxis in both uncapacitated and capacitated mouse sperm. Ejaculated sperm swim to the oviductal isthmus through the UTJ within 1–2 hr, but most require several more hours to reach the ampulla [ 6 ]. Song Analysis Of Shout By The Isley Brothers

Did Joseph Smith Break 10 Commandments Essay - Scale bars represent μm (C and D) and μm (E and F). (G) Positive rheotaxis is defined here as sperm movement against fluid flow within ±° of the forward vector of sperm movement. (H) Rates of rheotaxis for mouse and human sperm (mean ± SD) in five . Here, we report the first quantitative experimental study of mammalian sperm rheotaxis. Using microfluidic devices, we investigate systematically the swimming behavior of human and bull sperm over the whole range of physiologically relevant shear rates and viscosities. Our measurements show that the interplay of fluid shear, steric surface. El-sherry T, Abdel-Ghani M, Abou-Khalil N, Elsayed M, Abdelgawad M () Effect of pH on rheotaxis of bull sperm using microfluidics. Reprod Domest Anim – Article Google Scholar Fu HC, Powers TR, Stocker R () Bacterial rheotaxis. Proc . Marjane Satrapi: The Iranian Revolution

Apollo 11 Mission Rhetorical Analysis - thawed semen by rheotaxis could select superior bovine sperm subpopulations, and subsequent fertility was assessed and validated in vivo by artificial insemination of cows [4]. The selection of high quality spermatozoa prior to cryo-preservation is desirable. However, the major downside of microfluidic sperm selection technique is the limitation in. A sperm cell must complete a long and taxing journey to stand a chance of fertilising an egg cell. This quest covers a distance that is thousands of times longer than the length of a sperm cell. It also passes through the diverse environments of the cervix, the uterus and, finally, the oviduct, where there might be an egg to fertilise. How the sperm cells manage to stay on course over this Estimated Reading Time: 8 mins. For this purpose human sperm selected by rheotaxis were compared against density gradient, swim-up and a control group of non-selected spermatozoa in split frozen-thawed (FT) and fresh (F) semen samples. Sperm quality was assessed in terms of motility, morphology, DNA fragmentation index (DFI), viability, acrosome integrity and membrane fluidity. Research Paper On Malala Yousafzai

The Quietest People In To Kill A Mockingbird - Very recently, Rheotaxis, which is sperm cell tendency to swim against the flow, has been suggested as a possible mechanism for directing sperm cells to the oocyte when they swim against the fluid secreted in the female reproductive system after coitus. In this project we characterize rheotaxis of bull sperm using microfluidics. A sperm cell must complete a long and taxing journey to stand a chance of fertilising an egg cell. This quest covers a distance that is thousands of times longer than the length of a sperm cell. It also passes through the diverse environments of the cervix, the uterus and, finally, the oviduct, where there might be an egg to fertilise. How the sperm cells manage to stay on course over this. Effect of pH on rheotaxis of bull sperm using microfluidics, Reproduction In Domestic Animals, Ahmad Altayyeb, Maha Khashaba, Abdelhady Esmaeel, Mohamed El-mokhtar, Abdelbadei Sharkawy, Mohamed Abdelgawad And Essam Othman. Mechanical Characterization Of Endometrial Cells as a Non-Surgical Diagnostic Test For Endometriosis, The 21st. Native American Religion Summary

Jennifer L. Holms Boston Jane A Dream - Rheotaxis is a process whereby an organism swims against a flow. Rheotaxis is known in aquatic swimmers such as zebrafish and copepods (Shang et al., ). It has been discovered only recently that rheotaxis plays an important role in sperm migration (Miki and Clapham, ; El-Sherry et al., ; Tung et al., , a, b; Bukatin et al Estimated Reading Time: 11 mins. 2. The virtual sperm and its numerical simulation. Throughout the specification of a virtual sperm and the fluid dynamical equations, we use ξ to denote right-handed Cartesian coordinates fixed in a sperm flagellum reference frame, whose origin is located at the proximal end of the flagellum, with an associated orthonormal basis {e ξ i},.In addition, we define x = (x 1, x 2, x 3): = (x, y. Rheotaxis refers to sperm alignment according to the fluid flow, so that when the fluid flows against the sperm head it is called positive rheotaxis (Fig. 3A). Thermotaxis is sperm movement oriented by gradual differences in temperature towards the warmest place (Fig. 3B). patrick stump tattoos

Dworkins Theory Of Discretion - Bull sperm cells swimming in a low-viscosity fluid (3 mPa·s) near the wall of a planar channel. The video shows that, even at low viscosity, the flagellar beat of bull sperm is approximately planar. This follows from the fact that at certain instances the flagellum appears as a line (in contrast to human sperms at same viscosity, compare Video Estimated Reading Time: 10 mins. Sperm cells exhibiting positive rheotaxis behaviour align against the flow and spread through the channel [31, 32], reducing the likelihood of a sperm-sperm collision, and thus increasing the area available to the swimmers. The highly motile spermatozoa swim against the flow through the long channel (~ 30 mm) to reach the PCR. Cation channels of sperm (CatSper) are sperm-specific calcium channels with identified roles in the regulation of sperm function in humans, mice, and horses. We sought to employ a comparative genomics approach to identify conserved CATSPER genes in the bovine genome, and profile their expression in reproductive tissue. We hypothesized that CATSPER proteins expressed in bull testicular tissue. Janmar Coating Case Analysis

Christopher Columbuss Journal Analysis - Rheotaxis In Bull Sperm Words | 7 Pages. al () suggests that mechanical effects may exist through mechanosensing ion channels (MSCs) due to fluid shear stress increasing on the membrane of sperm head with an increase in the liquid velocity. MSCs are stretch activated channels that respond to mechanical stress on the cell membrane by. —Original Article— Involvement of calcium channels and intracellular calcium in bull sperm thermotaxis Md Anisuzzaman MONDAL 1), Yuji TAKAGI 1), Shoji A BABA2) and Koh-ichi HAMANO1) 1)Faculty of Agriculture, Shinshu University, Nagano , Japan 2)Faculty of Science, Ochanomizu University, Tokyo , Japan Abstract. Thermotaxis that sperm migrate. September Our manuscript on characterization of rheotaxis of bull sperm using microfluidics was accepted for publication in Integrative Biology (IF=) August Our manuscript on mammalian embryo vitrification using digital microfluidics was accepted in PLoS one (IF=). another word for outsider

Roberts V. Tuckey Case Study - Rheotaxis facilitates upstream navigation of mammalian sperm cells. A major puzzle in biology is how mammalian sperm determine and maintain the correct swimming direction during the various phases of the sexual reproduction process. Whilst chemotaxis is assumed to dominate in the immediate vicinity of the ovum, it is unclear which biochemical. Sperm guidance. Sperm guidance is the process by which sperm cells (spermatozoa) are directed to the oocyte (egg) for the aim of fertilization. In the case of marine invertebrates the guidance is done by chemotaxis. In the case of mammals, it appears to be done by chemotaxis, thermotaxis and rheotaxis. Emerging technologies in sperm selection: Can we solve ARTs frustration? SPERM SELECTION BASED ON MEMBRANE CHARACTERISTICS. Sperm selection is a significant step in ARTs execution. We have already discussed the practiced methods for quality sperm selection. Several studies claim that fragmented sperm cells are able to fertilize female eggs but result in defective embryos. The Meaning Of Human Identity In Dante Alighieris Divine Comedy

African American Inequality Essay - The mechanism that causes the detachment of spermatozoa from the oviductal reservoir around the time of ovulation remains to be elucidated. Because the cumulus cells of the bovine oocyte are known to secrete progesterone (P4), and P4 has been shown to act upon cation channels of spermatozoa (CatSper) in human spermatozoa, it was hypothesised that P4 could induce hyperactivation due to an. Characterization of rheotaxis of bull sperm using microfluidics. T El-Sherry, M Elsayed, H Abdelhafez, M Abdelgawad. Integrative Biology 6 (12), , Development of computer-assisted sperm analysis plugin for analyzing sperm motion in microfluidic environments using Image-J. The vagina is basically an elastic tube. It is the site for semen deposition in some species, such as cows (Bos taurus) and humans, but is bypassed in other species, such as pigs (Sus scrofa) [].Regardless, sperm that are destined to migrate up the tract spend little time in the vagina, because the acidity of vaginal fluid, which serves to kill bacterial pathogens, can also kill sperm [31,32]. starbucks marketing strategy

Similarities Between Macbeth And Dr Jekyll And Hyde - Characterization of rheotaxis of bull sperm using microfluidics. TM Elsherry, M Elsayed, HK Abdelhafez, M Abdelgawad. The 7th Cairo International Biomedical Engineering Conference (CIBEC ), Numerical investigation of multistage viscous micropump configurations. Gregorian Calendar Dbq Essay

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